美国MOBIO 土壤DNA提取试剂盒(UltraClean Soil DNA Isolation Kit)
UltraClean® Soil DNA Isolation KitOverviewFor isolating genomic DNA from routine soil and fecal samplesOrder | Description | Catalog # | Price |
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| 50 preps | 12800-50 | N/A | | | 100 preps | 12800-100 | N/A | |
Features- Established method selectively removes most PCR inhibitors - Rapidly purify DNA from up to one gram of soil in 30 minutes - Yields high quality PCR ready DNA up to 50 kb plus - Includes a proprietary Inhibitor Removal Solution to eliminate humic acid inhibitors of PCR - Efficiently lyses spores and fungi; also compatible with fecal samples DescriptionThe UltraClean® Soil DNA Isolation Kit has become the method of choice among researchers around the world studying microbial organisms in soil. This kit will isolate cellular, PCR quality DNA from soil and ensures removal of humic acid inhibitors.
Soil samples are added to a bead beating tube containing beads, lysis solution, bead solution and Inhibitor Removal Solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a vortex. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free Tris buffer.
This is our original DNA kit for soil - for more challenging soil samples we recommend the PowerSoil® DNA Isolation Kit NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System! Sample KitClick to try a sample. Research and Development
Purification of DNA from various soils using
the UltraClean® Soil DNA Isolation Kit
 Lane 1: Forest soil
Lane 2: Bog soil
Lane 3: Pristine sand
Lane 4: Farm soil PCR amplification of DNA extracted with this soil kit using universal eubacterial primers
 Lane 1: Marker
Lane 2: Negative control
Lanes 3-8: Six different soil types Learn more about the applications of the UltraClean® Soil DNA Isolation Kit in this poster presented at the American Society for Microbiology Meeting.
Testing the UltraClean® Soil DNA Isolation Kit
on a Diverse Range of Soils
by PCR Amplification of 16S rDNA. Download Here (pdf 872KB) Click over image to enlarge Specifications| Format | Silica Spin Filter Tubes |
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| Method | Bead Beating |
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| Binding Capacity | Up to 20 µg per filter |
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| Throughput | 1 - 24 samples |
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| Time | 30 minutes |
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| Equipment Required | Vortex Adapter |
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Positive PCR Results Obtained
with these Soil Types |
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- Clay
- Fecal/Intestinal Tract
- Fungal Mat
- Forest
| - Loam
- Sand
- Potting Soil
- Petroleum Saturated
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Publications- Andreote, F., Carneiro, R., Salles, J., Marcon, J., Labate, C., Azevedo, J., and Araujo, W. Culture-Independent Assessment of Rhizobiales-Related Alphaproteobacteria and the Diversity of Methylobacterium in the Rhizosphere and Rhizoplane of Transgenic Eucalyptus. Microbial Ecology. 57: 82-93 (2009).
- Baniulyte, D., Favila, E., and Kelly, J.Shifts in Microbial Community Composition Following Surface Application of Dredged River Sediment. Microbial Ecology. 57: 160-169 (2009).
- Barkovskii, A., Fukui, H., Leisen, J., Kim, S., Marsh, T., and Khijniak, A. Rearrangement of Bacterial Community Structure During Peat Diagenesis. Soil Biology and Biochemistry. 41: 135-143 (2009).
Customer CommentsI consulted a soil microbiologist for advice on a protocol for rapid DNA extraction from soil samples. He recommended the MO BIO UltraClean® Soil DNA Isolation Kit. My research has involved extensive soil sampling. The soil is analyzed for the presence of Streptomyces scabies, causal agent of potato scab, which is detectable by PCR. I have used the kit to process samples and have been extremely pleased with the results of not only getting consistent amounts of DNA, but also DNA that is high quality for PCR. I will continue using this kit because it is consistent and extremely quick. No other kit I have used is even half as fast.
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