美国MOBIO 土壤DNA大量提取试剂盒(UltraClean Mega Soil DNA Isolation Kit)
UltraClean® Mega Soil DNA Isolation KitOverviewFor isolating DNA from up to 10 grams of soilOrder | Description | Catalog # | Price |
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| 10 preps | 12900-10 | N/A | |
Features- Purifies DNA from 5 to 10 grams of soil in 50 minutes - Yields PCR ready DNA up to 50 kb plus - Includes a proprietary Inhibitor Removal Solution to eliminate humic acid inhibitors of PCR - Efficiently lyses spores and fungi and fecal samples - Ideal for samples with low microbial load - All tubes and reagents required are included in kit and ready to use DescriptionThe UltraClean® Mega Soil DNA Isolation Kit provides researchers the ability to process larger volumes of soil, critical when microbial load is low. This reliable kit is based on a bead beating technology that offers a proprietary Inhibitor Removal Solution to remove PCR inhibitors including humic acid.The UltraClean® Mega Soil DNA Isolation Kit has become the method of choice among researchers around the world studying microbial organisms in soil. This kit will isolate cellular, PCR quality DNA from soil and ensures removal of humic acid inhibitors. Our 50 ml Vortex Adapter for cell lysis and a centrifuge capable of spinning 50 ml tubes are required for maximum performance. Soil samples are added to a bead beating tube containing beads, lysis solution, bead solution and Inhibitor Removal Solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a vortex. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free Tris buffer. This is our original mega soil DNA kit - for more challenging 10 g soil samples we recommend the PowerMax® Soil DNA Isolation Kit. Research and DevelopmentPurification of DNA from various soils using
the UltraClean® Mega Soil DNA Isolation Kit
 Lane 1: Forest soil
Lane 2: Bog soil
Lane 3: Pristine sand
Lane 4: Farm soil PCR amplification of DNA extracted with this soil kit using universal eubacterial primers
 Lane 1: Marker
Lane 2: Negative control
Lanes 3-8: Six different soil types
Applications
Learn more about the applications of the UltraClean® Mega Soil DNA Isolation Kit in this poster presented at the American Society for Microbiology Meeting. Testing the UltraClean® Mega Soil DNA Isolation Kit on a Diverse Range of Soils by PCR Amplification of 16S rDNA. Download Here (pdf 872KB)  Click over image to enlarge Specifications| Format | Silica Spin Filter Tubes |
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| Method | Bead Beating |
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| Binding Capacity | Up to 1 mg per filter |
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| Sample Size | Up to 20 grams of soil |
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| Throughput | 1 - 8 samples |
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| Time | 50 minutes |
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| Equipment Required | Vortex and Vortex Adapters |
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Positive PCR Results Obtained
with these Soil Types | - Clay
- Fecal/Intestinal Tract
- Fungal mat
- Forest
| - Loam
- Sand
- Potting Soil
- Petroleum Saturated
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Storage ConditionsStore at room temperature for up to 2 years.Publications- Nunoura, T. and Takai, K. Comparison of Microbial Communities Associated with Phase-separation-induced Hydrothermal Fluids at the Yonaguni Knoll IV Hydrothermal Field, the Southern Okinawa Trough. FEMS Microbiology Ecology. 67(3): 351-370 (2009).
- Nunoura, T., Inagaki, F., Delwiche, M.E., Colwell, F.S., and Takai, K. Subseafloor Microbial Communities in Methane Hydrate-bearing Sediment at Two Distinct Locations (ODP Leg204) in the Cascadia Margin. Microbes and Environment. 23(4): 317-328 (2008).
- Fell, J.W., Scorzetti, G., Connell, L., and Craig, S. Biodiversity of Micro-eukaryotes in Antarctic Dry Valley Soils with <5% Soil Moisture. Soil Biology and Biochemistry. 38(10): 3107-3119 (2006).
Customer CommentsUltraClean® Mega Soil DNA Isolation Kit
I consulted a soil microbiologist for advice on a protocol for rapid DNA extraction from soil samples. He recommended the MO BIO UltraClean® Mega Soil DNA Isolation Kit. My research has involved extensive soil sampling. The soil is analyzed for the presence of Streptomyces scabies, causal agent of potato scab, which is detectable by PCR. I have used the kit to process samples and have been extremely pleased with the results of not only getting consistent amounts of DNA, but also DNA that is high quality for PCR. I will continue using this kit because it is consistent and extremely quick. No other kit I have used is even half as fast.
Elise C. Poole
Michigan State University
Dept. of Botany and Plant Pathology Read More Customer Comments
on the UltraClean® Mega Soil DNA Isolation Kit
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