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美国MOBIO 强力土壤DNA大量提取试剂盒(PowerMax Soil DNA Isolation Kit)




PowerMax® Soil DNA Isolation Kit

Overview

For efficiently isolating microbial DNA of high quality and purify from large quantities of soil - great for samples with low microbial load
Order
DescriptionCatalog #Price
10 preps12988-10N/A 

Features

- Purifies DNA from 10 g soil in 30 minutes - means NO time spent on multiple small sample processing

- Increases detection sensitivity on samples with a low microbial load

- Provides high quality DNA that is free of PCR inhibitors including humic substances

- Purified samples are suitable for PCR and QPCR

Description

The PowerMax® Soil DNA Isolation Kit is our next-generation kit designed for processing large quantities of any soil or environmental sample with high or low microbial load. Based on reagents from the PowerSoil® DNA Isolation Kit, the PowerMax® Soil DNA Isolation Kit uses our novel, patented Inhibitor Removal Technology® to remove PCR inhibiting compounds, including humic substances, and the brown color often associated with soil DNA. The kit is ideal for environmental samples that contain high humic substance content, including compost, sediment and manure. PCR analysis has been used to amplify DNA purified from microorganisms including Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces).

The PowerMax® Soil DNA Isolation Kit increases your ability to study microbial diversity in even the most difficult sample types, such as those which contain high humic levels or low microbial load. Our 50 ml Vortex Adapter for cell lysis and a centrifuge capable of spinning 50 ml tubes are required for maximum performance.

Sample Kit

Click to try a sample.

Research and Development

High Yield, Intact Genomic DNA

Total genomic DNA was isolated from 8 different soil samples using the PowerMax® Soil DNA Isolation Kit. 0.75 µg of each isolated DNA was analyzed on 1 % TAE agarose gel and ethidium stained. M = Marker DNA. Soil types are identified in the table below.


One microliter of total genomic DNA isolated using the PowerMax® Soil DNA Isolation Kit was PCR amplified using primers to the Bacillus spp. Amplified DNA was analyzed on 0.8% TAE agarose and ethidium stained. The amount of PCR fragment produced in each sample is representative of the relative amount of the template sequence in the purified DNA. Different soil sources will have different types and amount of organisms providing genomic DNA. N = Negative control lacking template. P = Positive control template. Soil types and amount are identified in the table below.


One microliter of total genomic DNA isolated using the PowerMax® Soil DNA Isolation Kit was PCR amplified using primers to the Streptococcus spp. DNA was analyzed on 1 % TAE agarose and ethidium stained. The amount of PCR fragment produced in each sample is representative of the relative amount of template sequence in the purified. Different soil sources will have different types and amount of organisms providing genomic DNA. N = Negative control lacking template. P = Positive control template. Soil types and amount are identified below.


Sample LaneTypeAmount processed (grams)
1Iowa corn field10
2So. California strawberry field10
3Cardiff estuary sediment10
4Carlsbad lagoon sediment10
5Home compost5
6San Diego City compost5
7Commercial potting mixture2.5
8Commercial peat moss2.5
PowerMax® Soil DNA Isolation Kit
Click over image to enlarge

MSDS

12988

Protocol

12988

Specifications

FormatSilica Spin Filter Tubes
MethodBead Beating
Sample SizeUp to 10 grams
Binding CapacityUp to 1 mg per filter
Throughput1 - 24 samples
Time30 minutes
Equipment RequiredVortex and Centrifuge
Soil Samples Tested
Aquifer SedimentManure
ClayMarine Sediment
Fecal Intestinal TractOil-saturated Soil
ForestPotting Soil
Fungal MatSand
LoamSewer Sludge

Storage Conditions

Store at room temperature for up to 2 years.

Publications


微生物菌种鉴定分析微生物组分析微生物检测分析 动植物基因组分析转录调控

   免费分析基因组dDDH值/ANI值

   真菌全基因组重测序

   细菌全基因重测序

   微生物新种鉴定分析

   微生物菌种多相鉴定分析

   酵母菌快速鉴定分析

   微生物序列分析

   菌群多样性组成谱全长测序

   菌群多样性组成谱测序

   宏基因组测序分析

   宏转录组测序分析

   标记基因全长测序

   扩增子/功能基因测序

   第三代高通量测序平台

   饲料检测与安全评价

   真菌基因组de novo测序

   细菌全基因组完成图测序

   真菌近完成图测序

   环境中活菌群分析

   产品微生物解析

   产品污染菌分析

   动植物基因组全长三代测序

   动植物基因组de novo测序

   SSR分子标记开发

   全基因组关联分析

   群体进化关系

   遗传图谱构建

   BSA性状定位

   免费分析基因组dDDH值/ANI值

   全长转录组测序

   全转录组测序

   转录组测序

   Small RNA测序

   SSR/STR/微卫星DNA技术

   扩增片段长度多态性(AFLP)


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