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DESCRIPTION

The Quick-RNA Whole Blood kit utilizes DNA/RNA Shield™, a unique preservation and lysis technology, to enable rapid isolation of total RNA from whole or partitioned blood or a cell pellet (after red blood cell lysis). The procedure uses Zymo-Spin column technology in which the sample is pre-filtered on the Zymo-Spin IIICG Column and then purified and concentrated on the Zymo-Spin IC Column. RNA is eluted into ≥ 6 µl of RNase-free water and is ready for any downstream application including RT-PCR, sequencing, etc.

HIGHLIGHTS

Superior Yields: Recover total RNA (including small/micro RNAs) without sample loss.
Protection: Worry-free blood sample storage at ambient temperatures for up to 30 days.
High-Quality: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-PCR, etc. DNase I Included.

TECHNICAL SPECIFICATIONS

Equipment	Microcentrifuge, vortex
Purity	RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source	Up to 1 ml mammalian whole-blood, plasma, or serum. Also compatible with pelleted blood cells (PBMCs, WBCs, buffy coat, pelleted sample from PAXgene™ Blood RNA Tube, etc.) and nucleated blood.
Size Range	Total RNA ≥ 17 nt
Yield	10 µg RNA (binding capacity), ≥6 µl (elution volume)