美国ZYMO RESEARCH-ZR small-RNA PAGE Recovery Kit R1070
DESCRIPTION
The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the "crush and soak" method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations. HIGHLIGHTS
- Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- Compatible with up to 25% (w/v) polyacrylamide.
TECHNICAL SPECIFICATIONS
| Equipment | Microcentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer |
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| Purity | RNA is ready for all subsequent analysis and molecular manipulation |
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| Sample Source | RNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar). |
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| Size Range | RNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery) |
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| Yield | 10 µg RNA (binding capacity), ≥6 µl (elution volume) |
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| Cat # | Name | Size |
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| R1070 | ZR small-RNA PAGE Recovery Kit | 20 Preps |
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